
Thermo Fisher Scientific Bcl-2 (Apoptosis and Follicular Lymphoma Marker) Monoclonal Antibody (124)
Bcl-2 단백질을 표적하는 마우스 모노클로날 항체로, 인간 시료에 반응하며 apoptosis 및 follicular lymphoma 연구에 적합합니다. Western blot, IHC, ICC/IF, Flow cytometry 등 다양한 응용에 사용 가능합니다. 4°C 보관, Protein A/G 정제, PBS buffer 포함.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 124 |
| Immunogen | Synthetic peptide aa41–54 (GAAPAPGIFSSQPG-Cys) of human Bcl-2 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Does not react with Mouse, Rat, Others-not known.
Target Information
BCL-2 is a key regulator of apoptosis that can inhibit or promote cell death. Members of the BCL-2 family dimerize to modulate apoptosis. Bag1 forms a heterodimer with BCL-2, enhancing its anti-apoptotic effect. Bax and Bak are critical for cytochrome c release from mitochondria, initiating apoptosis. Constitutive expression of BCL-2, such as translocation to the Ig heavy chain locus, is associated with follicular lymphoma. In most follicular lymphomas, neoplastic germinal centers express high levels of BCL-2 alpha protein, while normal centers are negative. Overexpression of BCL-2 has been linked to cancers such as B-cell lymphoma and prostate cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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