Thermo Fisher Scientific BCKDHA Polyclonal Antibody
BCKDHA 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, ICC/IF 등 다양한 응용에 적합함. 인간, 생쥐, 랫트 반응성. 고순도 Affinity 정제, PBS+50% glycerol 보존. 연구용으로만 사용 가능.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human BCKDHA (Accession P12694), corresponding to amino acid residues A254–F304 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2899533 |
Product Specific Information
Antibody detects endogenous levels of total BCKDHA.
Target Information
The BCKDHA gene encodes the E1-alpha subunit of the branched-chain alpha-keto acid (BCAA) dehydrogenase complex (BCKD; EC 1.2.4.4), an inner-mitochondrial enzyme complex that catalyzes the oxidative decarboxylation of branched-chain alpha-ketoacids derived from isoleucine, leucine, and valine.
This reaction is the second major step in the catabolism of branched-chain amino acids. The BCKD complex consists of three catalytic components:
- A heterotetrameric (α2–β2) branched-chain α-keto acid decarboxylase (E1)
- A homo-24-meric dihydrolipoyl transacylase (E2)
- A homodimeric dihydrolipoamide dehydrogenase (E3)
E1 is a thiamine pyrophosphate (TPP)-dependent enzyme, and the reaction is irreversible, constituting the first committed step in BCAA oxidation. The BCKDHB gene encodes the beta subunit of E1. The complex also contains two regulatory enzymes, a kinase and a phosphorylase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지: PA5-114897_BCKDHA_P12694-1_Rabbit.svg, PA5-114897_BCKDHA_P12694-1_Rabbit_PDP.jpeg)
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