Thermo Fisher Scientific RAG2 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 258-527 of human RAG2 (NP_0012307151) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:RAG2,uniProtId:P55895-1,ncbiNodeId:9606,antigenRange:258-527,antigenLength:527,antigenImageFileName:PA5-88580_RAG2_P55895-1_Rabbit.svg,antigenImageFileNamePDP:PA5-88580_RAG2_P55895-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.7 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.09% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2805011

Product Specific Information

Positive Samples: Mouse testis, Mouse liver; Cellular Location: Nucleus

Immunogen sequence: VSSAILTQTN NDEFVIVGGY QLENQKRMIC NIISLEDNKI EIREMETPDW TPDIKHSKIW FGSNMGNGTV FLGIPGDNKQ VVSEGFYFYM LKCAEDDTNE EQTTFTNSQT STEDPGDSTP FEDSEEFCFS AEANSFDGDD EFDTYNEDDE EDESETGYWI TCCPTCDVDI NTWVPFYSTE LNKPAMIYCS HGDGHWVHAQ CMDLAERTLI HLSAGSNKYY CNEHVEIARA LHTPQRVLPL KKPPMKSLRK KGSGKILTPA KKSFLRRLFD

Target Information

RAG2 (V(D)J recombination-activating protein 2) is a core component of the RAG complex - a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T-lymphocytes through rearrangement of different V (variable), in some cases D (diversity) and J (joining) gene segments. DNA cleavage by the RAG complex occurs in 2 steps: 1) first a nick is introduced in the top strand immediately upstream of the heptamer, generating a 3-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends. 2) hairpin coding ends and 2 blunt, 5-phosphorylated ends are created. The chromatin structure plays an essential rold in the V(D)J recombination reactions and the presence of histone H3 trimethylated at Lys-4 (H3K4me3) stimulates both the nicking and hair pinning expressed on individual B-lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin. This prevents accessibility to the RAG complex and recombination of the second allele. In the RAG complex, RAG2 is not the catalytic component but is required for all known catalytic activities mediated by RAG1. Mutations affecting the gene can results in combined cellular and humoral immune defects with granulomas, severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-negative/NK-cell-positive, and Omenn syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.