Thermo Fisher Scientific VAMP2 Polyclonal Antibody
Rabbit polyclonal antibody against human VAMP2 for WB, IHC, and ELISA. Recognizes human, mouse, and rat. Affinity purified, liquid form, stored at -20°C. Suitable for research on vesicle membrane fusion and synaptic function.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 |
| ELISA | 1 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 1–100 of human VAMP2 (NP_055047.2) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.42 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2805013 |
Product Specific Information
- Immunogen sequence:
MSATAATAPP AAPAGEGGPP APPPNLTSNR RLQQTQAQVD EVVDIMRVNV DKVLERDQKL SELDDRADAL QAGASQFETS AAKLKRKYWW KNLKMMIILG - Positive Samples: 22Rv1, Mouse brain, Mouse lung, Rat brain
- Cellular Location: Cell junction, Cell membrane, Cytoplasmic vesicle, Single-pass type IV membrane protein, Secretory vesicle, Synapse, Synaptic vesicle membrane, Synaptosome
Target Information
The vesicle-associated membrane proteins (VAMP) or synaptobrevins are calcium-binding proteins specific to eukaryotes. VAMPs, along with SNAP-25 and syntaxin, form the core complex of soluble NSF attachment protein receptor (SNARE) proteins that interact with NSF and alpha-SNAP. These membrane-associated proteins play a key role in regulating vesicle membrane fusion with the plasma membrane. The Clostridium tetani neurotoxin is a metalloprotease specific for VAMP. In Alzheimer’s disease, VAMP levels of all isoforms appear significantly lowered. VAMP2 is suggested to be a resident protein of the insulin-sensitive glucose transporter type 4 (GLUT4) compartment and is required for GLUT4 vesicle incorporation into the cell surface in response to insulin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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