Thermo Fisher Scientific CD11b Monoclonal Antibody (M1/70), Functional Grade, eBioscience
CD11b 단백질을 인식하는 M1/70 클론의 Rat 모노클로날 항체로, Flow Cytometry 및 IHC(Frozen)에 최적화됨. Functional Grade 등급으로 세포 기능 분석 및 중화 실험에 적합. Mouse와 Human 시료 모두에 반응하며 높은 순도(>90%)와 낮은 엔도톡신 수준을 보유.
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- 16-0112-xxx (6개 옵션)
- 판매단위
- pk
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Thermo Fisher Scientific CD11b Monoclonal Antibody (M1/70), Functional Grade, eBioscience™
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 4 publications |
| Immunohistochemistry (IHC) | - | View 48 publications |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | - | View 6 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | View 3 publications |
| Immunocytochemistry (ICC/IF) | - | View 17 publications |
| Flow Cytometry (Flow) | 0.25 µg/test | View 283 publications |
| Immunoprecipitation (IP) | - | View 1 publication |
| Neutralization (Neu) | Assay-Dependent | View 2 publications |
| Functional Assay | Assay-Dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Published Species | Fish, Human, Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), Functional Grade |
| Class | Monoclonal |
| Type | Antibody |
| Clone | M1/70 |
| Conjugate | Functional Grade |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2865615 |
Available Conjugate Formats
- Unconjugated
- Alexa Fluor 488, 532, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV Series (395–805)
- Brilliant Violet Series (421–786)
- eFluor 450, 506, 660
- FITC
- NovaFluor Blue/Yellow Series
- PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright Series (436–780)
- Custom conjugation available upon request
Product Specific Information
Description:
The M1/70 monoclonal antibody reacts with mouse CD11b (integrin alphaM, 165–170 kDa). CD11b forms a non-covalent complex with CD18 (Mac-1) and binds CD54 (ICAM-1), C3bi, and fibrinogen. It is expressed by macrophages, NK cells, granulocytes, activated lymphocytes, and B-1 cells. M1/70 also cross-reacts with human CD11b and detects this antigen on monocytes, granulocytes, and NK cells. CD11b participates in adhesive cell interactions through ligand binding.
Applications Reported:
- Flow cytometric analysis
- Immunohistochemical staining of frozen sections
- In vitro blocking of CD11b function
- (Not recommended for formalin-fixed paraffin-embedded tissue)*
Applications Tested:
- Tested in flow cytometric analysis of mouse splenocytes or bone marrow cells
- Recommended ≤ 0.25 µg/test (100 µL final volume, 10⁵–10⁸ cells/test)
- Optimize antibody titration for best assay performance
Storage and Handling:
- Use in sterile environment
- 0.2 µm post-manufacturing filtered
- Purity >90% (SDS-PAGE)
- Endotoxin <0.001 ng/µg (LAL assay)
- Aggregation <10% (HPLC)
Target Information
CD11b (integrin alpha-M, ITGAM) is a 165 kDa adhesion molecule forming a heterodimer with CD18 (integrin beta-2). The CD11b/CD18 complex, known as Mac-1 or CR3, acts as a receptor for ICAM-1, ICAM-2, ICAM-4, CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X.
It is expressed on monocytes/macrophages, granulocytes, activated lymphocytes, NK cells, dendritic cells, and brain microglia. CD11b expression increases during monocyte maturation and varies across tissue macrophages.
Associated diseases include systemic lupus erythematosus and ITGAM-related lupus susceptibility.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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