Thermo Fisher Scientific CD16 Monoclonal Antibody (eBioCB16 (CB16)), Functional Grade, eBioscience

Thermo Fisher Scientific CD16 Monoclonal Antibody (eBioCB16 (CB16)), Functional Grade, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 661 / 737 / 805
• Brilliant Violet 480
• eFluor 450 / 506
• FITC
• NovaFluor Blue 610-70S / 660-120S
• NovaFluor Red 710 / 725
• NovaFluor Yellow 660 / 730
• PE / PE-Cyanine5 / PE-Cyanine7 / PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436 / 600 / 645 / 702 / 780
• Request custom conjugation available

Product Specific Information

The eBioCB16 monoclonal antibody recognizes CD16 (FcγRIII), a low-affinity receptor for IgG with a molecular weight of 50–80 kDa.
CD16 includes two similar genes:

• CD16A (FcγRIIIA) – expressed on macrophages and NK cells
• CD16B (FcγRIIIB) – expressed on neutrophils

Two polymorphisms (NA-1 and NA-2) exist for CD16B; individuals homozygous for NA-2 exhibit lower phagocytic capacity than NA-1. CD16 binds IgG immune complexes, preferentially IgG1 and IgG3, and mediates ADCC, phagocytosis, degranulation, and proliferation upon activation.

Applications Reported: Suitable for flow cytometric analysis.
Applications Tested: Validated on normal human peripheral blood cells at ≤0.5 µg/test (100 µL final volume). Cell count range: 10⁵–10⁸ cells/test. Titrate for optimal performance.

Storage and Handling: Use in sterile conditions.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: >90% (SDS-PAGE).
Endotoxin Level: <0.001 ng/µg antibody (LAL assay).
Aggregation: <10% (HPLC).

Target Information

CD16 encodes a receptor that binds the Fc region of IgG and is involved in immune complex clearance, antibody-dependent cytotoxicity, and modulation of viral infections.

• Gene: FCGR3A
• Related Gene: FCGR3B (chromosome 1)
• Expression:
  • FCGR3A – NK cells (transmembrane anchoring)
  • FCGR3B – neutrophils (PI linkage)

Mutations in FCGR3A are associated with Immunodeficiency 20, Herpes Zoster, and Systemic Lupus Erythematosus susceptibility. Alternative splicing produces multiple isoforms.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.