Thermo Fisher Scientific CD16 Monoclonal Antibody (eBioCB16 (CB16)), Functional Grade, eBioscience
CD16(FcγRIII) 인식하는 eBioCB16 단클론 항체로, 인간 및 마우스 시료에 적합합니다. Flow Cytometry 등 다양한 기능성 분석에 사용 가능하며, 1 mg/mL 농도의 액상 형태로 제공됩니다. 높은 순도(>90%)와 낮은 엔도톡신 수준(<0.001 ng/µg)을 보장합니다.
- 카탈로그번호
- 16-0168-xxx (3개 옵션)
- 판매단위
- pk
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3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD16 Monoclonal Antibody (eBioCB16 (CB16)), Functional Grade, eBioscience
Thermo Fisher Scientific CD16 Monoclonal Antibody (eBioCB16 (CB16)), Functional Grade, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | - | View 2 publications |
| Flow Cytometry (Flow) | 0.5 µg/test | View 30 publications |
| Functional Assay | Assay-Dependent | View 1 publication |
| Control | Assay-Dependent | - |
| T-Cell Activation (TCA) | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Functional Grade, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioCB16 (CB16) |
| Conjugate | Functional Grade |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2865642 |
Available Formats
- Unconjugated
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 661 / 737 / 805
- Brilliant Violet 480
- eFluor 450 / 506
- FITC
- NovaFluor Blue 610-70S / 660-120S
- NovaFluor Red 710 / 725
- NovaFluor Yellow 660 / 730
- PE / PE-Cyanine5 / PE-Cyanine7 / PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436 / 600 / 645 / 702 / 780
- Request custom conjugation available
Product Specific Information
The eBioCB16 monoclonal antibody recognizes CD16 (FcγRIII), a low-affinity receptor for IgG with a molecular weight of 50–80 kDa.
CD16 includes two similar genes:
- CD16A (FcγRIIIA) – expressed on macrophages and NK cells
- CD16B (FcγRIIIB) – expressed on neutrophils
Two polymorphisms (NA-1 and NA-2) exist for CD16B; individuals homozygous for NA-2 exhibit lower phagocytic capacity than NA-1. CD16 binds IgG immune complexes, preferentially IgG1 and IgG3, and mediates ADCC, phagocytosis, degranulation, and proliferation upon activation.
Applications Reported: Suitable for flow cytometric analysis.
Applications Tested: Validated on normal human peripheral blood cells at ≤0.5 µg/test (100 µL final volume). Cell count range: 10⁵–10⁸ cells/test. Titrate for optimal performance.
Storage and Handling: Use in sterile conditions.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: >90% (SDS-PAGE).
Endotoxin Level: <0.001 ng/µg antibody (LAL assay).
Aggregation: <10% (HPLC).
Target Information
CD16 encodes a receptor that binds the Fc region of IgG and is involved in immune complex clearance, antibody-dependent cytotoxicity, and modulation of viral infections.
- Gene: FCGR3A
- Related Gene: FCGR3B (chromosome 1)
- Expression:
- FCGR3A – NK cells (transmembrane anchoring)
- FCGR3B – neutrophils (PI linkage)
Mutations in FCGR3A are associated with Immunodeficiency 20, Herpes Zoster, and Systemic Lupus Erythematosus susceptibility. Alternative splicing produces multiple isoforms.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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