Thermo Fisher Scientific CD16 Monoclonal Antibody (3G8), Functional Grade, eBioscience

Thermo Fisher Scientific CD16 Monoclonal Antibody (3G8), Functional Grade, eBioscience

Applications and Tested Dilutions

Publications

• Flow Cytometry: View 4 publications
• Functional Assay: View 1 publication

Product Specifications

Available Conjugate Formats

• APC-eFluor 780
• NovaFluor Blue 510
• NovaFluor Blue 530
• NovaFluor Blue 585
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 660-40S
• NovaFluor Blue 760
• NovaFluor Red 685
• NovaFluor Red 700
• NovaFluor Red 725
• NovaFluor Red 755
• NovaFluor Yellow 590
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• NovaFluor Yellow 690
• NovaFluor Yellow 700
• NovaFluor Yellow 730
• NovaFluor Yellow 755
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Request custom conjugation

Product Specific Information

This 3G8 monoclonal antibody reacts with human and non-human primate CD16 (Fc gamma RIII), which exists as two isoforms, FcγRIIIA and FcγRIIIB.

• FcγRIIIA: Expressed on monocytes, macrophages, and NK cells
• FcγRIIIB: Expressed on neutrophils

In non-human primates, CD16 expression patterns vary by species. Binding of IgG triggers signal transduction leading to ADCC, phagocytosis, cytokine release, and antigen presentation.

The 3G8 clone exhibits functional activities such as inhibition of cytotoxic ability, activation of cell signaling, and NK cell depletion in vivo. It has been validated in multiple primate species (e.g., chimpanzee, rhesus, marmoset, baboon).

Cross-blocking studies show 3G8 recognizes the same epitope as CB16, but distinct from B73.1.

Applications Reported: Flow cytometric analysis and functional assays
Applications Tested: Flow cytometric analysis of normal human peripheral blood cells (≤1 µg/test, 100 µL final volume)

Filtration: 0.2 µm post-manufacturing filtered
Purity: >90% (SDS-PAGE)
Endotoxin Level: <0.001 ng/µg (LAL assay)
Aggregation: <10% (HPLC)

Target Information

CD16 encodes a receptor recognizing the Fc portion of IgG, involved in clearance of immune complexes and mediation of cytotoxicity.

• Gene: FCGR3A (chromosome 1)
• Expression: NK cells (transmembrane anchoring), neutrophils (PI linkage via FCGR3B)
• Associated Diseases: Immunodeficiency 20, Herpes Zoster, SLE, alloimmune neonatal neutropenia
• Alternative splicing yields multiple isoforms.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.