Thermo Fisher Scientific CD14 Monoclonal Antibody (61D3), Functional Grade, eBioscience

Thermo Fisher Scientific CD14 Monoclonal Antibody (61D3), Functional Grade, eBioscience™

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395–805 series
• Brilliant Violet 421–786 series
• eFluor 450, 506
• FITC
• NovaFluor Blue & Yellow series
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436–780 series
• Request custom conjugation

Product Specific Information

The 61D3 monoclonal antibody reacts with human CD14, a 53–55 kDa GPI-linked glycoprotein expressed on monocytes, interfollicular macrophages, and some dendritic cells. Complexes of LPS and LBP bind with high affinity to monocytes through surface CD14.

Applications Reported:

• Flow cytometric analysis
• In vitro functional studies (fluorochrome-conjugated 61D3 recommended for flow cytometry)

Applications Tested:

• Tested by flow cytometric analysis of normal human peripheral blood cells
• ≤1 µg/test (100 µL final volume; 10⁵–10⁸ cells/test)
• Antibody titration recommended for optimal assay performance

Storage and Handling:

• Use in a sterile environment
• 0.2 µm post-manufacturing filtered
• Purity >90% (SDS-PAGE)
• Endotoxin <0.001 ng/µg (LAL assay)
• Aggregation <10% (HPLC)

Target Information

CD14 is a 55 kDa GPI-anchored glycoprotein expressed on mature monocytes, macrophages, and neutrophils. It acts as a multifunctional lipopolysaccharide receptor, found both in membrane-bound and soluble forms. CD14 binds LPS via LBP, discriminates structural differences between LPS molecules, and plays roles in inflammatory and infectious responses. It can also bind apoptotic cells and influence allergic reactions.
Diseases associated with CD14 dysfunction include Mycobacterium chelonae infection and Croup.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.