Thermo Fisher Scientific CD16/CD32 Monoclonal Antibody (93), Functional Grade, eBioscience

Thermo Fisher Scientific CD16/CD32 Monoclonal Antibody (93), Functional Grade, eBioscience™

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• Biotin
• Brilliant UV 496
• Brilliant UV 661
• eFluor 450
• FITC
• NovaFluor Red 685
• NovaFluor Red 700
• NovaFluor Red 710
• NovaFluor Red 755
• NovaFluor Yellow 690
• PE
• PE-Cyanine7
• PerCP-Cyanine5.5
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Request custom conjugation

Product Specific Information

Description:
The 93 monoclonal antibody reacts with an epitope shared by mouse CD16 and CD32. CD16 (FcγRIII) and CD32 (FcγRII) are low-affinity receptors for the mouse IgG Fc portion and are expressed by B cells, monocyte/macrophages, NK cells, and neutrophils.

Applications Reported:

• Flow cytometric analysis
• Blocking of Fc-mediated reactions in functional studies

Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes. Recommended ≤0.5 µg per test (100 µL final volume). Cell number may vary from 10⁵–10⁸ cells/test. Antibody should be titrated for optimal performance.

Storage and Handling:
Use in sterile environment. Store at 4°C.

Filtration:
0.2 µm post-manufacturing filtered.

Purity:

90%, determined by SDS-PAGE.

Endotoxin Level:
<0.001 ng/µg antibody (LAL assay).

Aggregation:
<10%, determined by HPLC.

Target Information

CD16 is a 50–65 kDa cell surface molecule existing in two forms: a transmembrane form (NK cells, some T cells) and a phosphatidylinositol-linked form (granulocytes). It serves as a low-affinity receptor for IgG (FcRIII) and mediates ADCC by NK cells.

CD32 is a 40 kDa glycoprotein acting as a low-affinity receptor for IgG (FcγRII). It mediates endocytosis, secretion activation, cytotoxicity, and immunomodulation. Expressed by B cells, monocytes, granulocytes, and platelets. CD32 participates in immune complex phagocytosis and B-cell antibody regulation. Genetic variations of CD32 may increase susceptibility to systemic lupus erythematosus (SLE). Multiple transcript variants encoding different isoforms are known.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.