Thermo Fisher Scientific SIP1 Polyclonal Antibody
Thermo Fisher Scientific의 SIP1 Polyclonal Antibody는 ZEB2(Smad interacting-protein 1)를 인식하는 Rabbit IgG 항체로, WB, IHC, IP 등 다양한 응용에 적합합니다. 고순도 항원 친화 크로마토그래피로 정제되었으며 인체 및 마우스 반응성이 예측됩니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1:2,000–1:10,000
Immunohistochemistry (IHC)
- Tested Dilution: 1:100–1:500
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:100–1:500
Immunoprecipitation (IP)
- Tested Dilution: 2–5 µg/mg lysate
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 1164 and 1214 of Human Smad interacting-protein 1 (ZEB2, UniProt ID: O60315-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS, pH 7.0–8.0, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections
- Based on 100% sequence identity, this antibody is predicted to react with Mouse
Target Information
ZEB2, also known as Smad interacting-protein 1, is a nuclear DNA-binding transcriptional repressor that interacts with activated SMADs. Similar to ZEB1, ZEB2 inhibits E-cadherin transcription and induces epithelial-mesenchymal transition (EMT), which regulates cell migration during embryonic development and wound healing. ZEB2 may protect cells from DNA damage-induced apoptosis, contributing to tumor progression. It is often found in the cytoplasm of various cancer tissues, suggesting localization regulation. Mutations in ZEB2 are associated with Hirschsprung disease and Mowat-Wilson syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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