Thermo Fisher Scientific SIP1 Polyclonal Antibody
인간 ZEB2(Smad interacting-protein 1)에 특이적인 Rabbit Polyclonal 항체로, WB, IHC, IP 등 다양한 응용에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, 액상 형태로 제공. 4°C 보관, 1년의 권장 보관 기간.
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Thermo Fisher Scientific SIP1 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 750 and 800 of Human Smad interacting-protein 1 (ZEB2, UniProt ID: O60315-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Tris citrate/phosphate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Information
ZEB2, also known as Smad interacting-protein 1, is a DNA-binding transcriptional repressor that interacts with activated SMADs. It inhibits transcription of the E-cadherin gene and induces epithelial-mesenchymal transition (EMT), a process controlling cell migration during development and wound healing. ZEB2 also protects cells from DNA damage-induced apoptosis, potentially contributing to tumor progression. It is often observed in the cytoplasm of cancer tissues, suggesting regulated localization. Mutations in ZEB2 are associated with Hirschsprung disease and Mowat-Wilson syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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