Thermo Fisher Scientific CD197 (CCR7) Monoclonal Antibody (4B12), APC, eBioscience
마우스 CCR7(CD197)을 인식하는 단클론 항체로, APC 형광 표지되어 흐름세포분석에 최적화됨. 4B12 클론은 림프구 이동 및 면역 반응 연구에 유용하며, 37°C에서 더 밝은 염색 결과를 제공. 연구용으로만 사용 가능.
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Thermo Fisher Scientific CD197 (CCR7) Monoclonal Antibody (4B12), APC, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Flow Cytometry (Flow) | 1 µg/test | View 29 publications |
| Immunoprecipitation (IP) | - | View 1 publication |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), APC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4B12 |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_469443 |
Available Formats
- Alexa Fluor 700
- APC-eFluor 780
- Biotin
- eFluor 450
- Functional Grade
- PE
- PE-Cyanine7
- PE-eFluor 610
- PerCP-Cyanine5.5
- Request custom conjugation
Product Specific Information
The 4B12 monoclonal antibody reacts with mouse CCR7 (CD197), a chemokine receptor for CCL19 and CCL21. CCR7 mediates lymphocyte migration to secondary lymphoid organs and plays roles in dendritic cell and B cell localization during immune responses.
Human CCR7 is also a marker for central memory T cells (TCM), expressed with CD62L, and is involved in thymocyte development. CCR7 deficiency leads to abnormal thymic architecture and T cell development.
For optimal CCR7 visualization, multi-color staining and incubation at 37°C are recommended for brighter fluorescence and improved resolution. The 4B12 antibody has been tested in flow cytometric analysis of mouse splenocytes and thymocytes at ≤1 µg/test.
Recommended Staining Protocol
- Prepare cell suspension and block FcγIII R/FcγII R with 5 µg/million cells anti-mouse CD16/32 for 15 min on ice.
- Without washing, add 1 µg/million cells 4B12 and incubate at 37°C (or 2–8°C) for 30 min.
- Wash cells once with 3 mL Flow Cytometry Staining Buffer and decant supernatant.
- Analyze by flow cytometry or proceed with secondary staining on ice.
Note: Co-staining with 4B12 and CCL19-Fc may be challenging due to steric hindrance and differing binding conditions. 4B12 binding can block detectable CCL19-Fc binding.
Excitation: 633–647 nm
Emission: 660 nm
Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CCR7 is a G protein-coupled chemokine receptor induced by Epstein–Barr virus (EBV) and expressed in blood, bone marrow, lymph node, and intestine. It regulates T cell chemotaxis, dendritic cell maturation, and migration of memory T cells to inflamed tissues. Ligands include CCL19 and CCL21.
Receptors of the C-C chemokine family include CCR1–CCR5 and Duffy antigen, which are essential for immune cell migration and inflammation response.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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