Thermo Fisher Scientific ROCK1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 400-660 of human ROCK1 (NP_0053971) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ROCK1,uniProtId:Q13464-1,ncbiNodeId:9606,antigenRange:400-660,antigenLength:1354,antigenImageFileName:PA5-87623_ROCK1_Q13464-1_Rabbit.svg,antigenImageFileNamePDP:PA5-87623_ROCK1_Q13464-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.1 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2804298

Product Specific Information

Immunogen sequence: YSNRRYLSSA NPNDNRTSSN ADKSLQESLQ KTIYKLEEQL HNEMQLKDEM EQKCRTSNIK LDKIMKELDE EGNQRRNLES TVSQIEKEKM LLQHRINEYQ RKAEQENEKR RNVENEVSTL KDQLEDLKKV SQNSQLANEK LSQLQKQLEE ANDLLRTESD TAVRLRKSHT EMSKSISQLE SLNRELQERN RILENSKSQT DKDYYQLQAI LEAERRDRGH DSEMIGDLQA RITSLQEEVK HLKHNLEKVE GERKEAQDML N; Positive Samples: 293T, U-251MG, Jurkat, BT-474; Cellular Location: Cell membrane, Cell projection, Cytoplasm, Golgi apparatus membrane, Peripheral membrane protein, bleb, centriole, centrosome, cytoskeleton, lamellipodium, microtubule organizing center, ruffle

Target Information

ROCK1 is a serine/threonine kinase that regulates cell differentiation and migration. The Rho/ROCK pathway is involved in the progression of various human cancers. ROCK1 has been found to be a new Caspase-3 substrate. ROCK, one of the effectors of the small GTPase Rho, has recently been shown to contribute significantly to myosin light chain (MLC) activation through two pathways: direct phosphorylation of MLC and phosphorylation of MLC phosphatase, leading to its inhibition. The increase in cellular contractility that is necessary for apoptotic membrane blebbing implies sustained augmentation of MLC phosphorylation. ROCK-1 consists of an amino-terminal kinase domain and an inhibitory cysteine/histidine-rich C-terminal domain that is located within a pleckstrin-homology region. They are joined by a variable region that contains the Rho-binding domain. During apoptosis, ROCK-I is cleaved by caspase-3 at a conserved DETD1113/G sequence and its carboxy-terminal inhibitory domain is removed, resulting in deregulated and constitutive kinase activity. The caspase-3-mediated cleavage and activation of ROCK I induces phosphorylation of MLC and membrane blebbing, one of the first events in the execution phase of apoptosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.