Thermo Fisher Scientific CD53 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 100-180 of human CD53 (NP_0005511) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CD53,uniProtId:P19397-1,ncbiNodeId:9606,antigenRange:100-180,antigenLength:219,antigenImageFileName:PA5-87622_CD53_P19397-1_Rabbit.svg,antigenImageFileNamePDP:PA5-87622_CD53_P19397-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.09 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2804297

Product Specific Information

Immunogen sequence: AILLFVYEQK LNEYVAKGLT DSIHRYHSDN STKAAWDSIQ SFLQCCGING TSDWTSGPPA SCPSDRKVEG CYAKARLWFH S; Positive Samples: B cells, Jurkat, Raji, HL-60, Mouse liver, Mouse spleen, Mouse lung; Cellular Location: Cell junction, Cell membrane, Membrane, Multi-pass membrane protein

Target Information

CD53 is a tetraspanin family transmembrane glycoprotein expressed in the lymphoid-myeloid lineage. This molecule has been reported to form complexes with other leukocyte surface proteins such as CD2, CD19, CD21, MHC II, VLA-4 or tetraspanins CD37, CD81 and CD82, thus probably modulating various signaling processes. CD53 is involved in radioresistancy of tumor cells and its triggering has anti-apoptotic effect. In thymus, CD53 is up-regulated in response to positive selection signals during T cell development, and is strongly expressed upon macrophage exposure to bacterial lipopolysaccharide, whereas stimulation of neutrophils results in down-regulation of CD53 expression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.