Thermo Fisher Scientific SERCA2 ATPase Monoclonal Antibody (IID8)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:5,000

View 85 publications 85 publications

Immunohistochemistry (IHC)

-

View 19 publications 19 publications

Immunohistochemistry (Paraffin) (IHC (P))

1 µg/mL

-

Immunohistochemistry (Frozen) (IHC (F))

1:500

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

5 µg/mL

View 31 publications 31 publications

Flow Cytometry (Flow)

1 µg / 10^6 cells

-

ELISA (ELISA)

-

View 1 publication 1 publication

Immunoprecipitation (IP)

-

View 4 publications 4 publications

ChIP assay (ChIP)

-

View 1 publication 1 publication

in situ PLA (PLA)

-

View 1 publication 1 publication

Immunomicroscopy (IM)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Dog, Human, Mouse, Pig, Rabbit, Rat

Published species

Amphibian, Dog, Guinea pig, Hamster, Human, Mouse, Non-human primate, Pig, Rabbit, Rat, Sheep, Virus, Zebrafish

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

IID8

Immunogen

Purified canine cardiac sarcoplasmic reticulum. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:SERCA2 ATPase,uniProtId:O46674-1,ncbiNodeId:9615,antigenRange:1-997,antigenLength:997,antigenImageFileName:MA3-910_SERCA2_ATPase_O46674-1_House_mouse.svg,antigenImageFileNamePDP:MA3-910_SERCA2_ATPase_O46674-1_House_mouse_PDP.jpeg,sortOrder:3}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2227681

Product Specific Information

MA3-910 detects sarcoplasmic or endoplasmic reticulum calcium 2 (SERCA2) ATPase from bovine, canine, human, pig, rabbit and rat tissues. This antibody does not detect frog SERCA2 ATPase (J. Biol. Chem., Feb 2001; 276: 3911 - 3919). MA3-910 detects SERCA2 ATPase and does not discriminate between the two isoforms.

MA3-910 has been successfully used in Western blot, immunofluorescence, immunocytochemistry, and immunohistochemistry. By Western blot, this antibody detects an ~110 kDa protein representing SERCA2 ATPase from canine skeletal muscle triad preparations. Immunofluorescence staining of SECRA2 ATPase in rabbit skeletal muscle with MA3-910 results in strong labeling of the entire type I (slow) myofiber consistent with sarcoplasmic reticulum localization.

MA3-910 is not recommended for Western blot detection of rat SERCA2.

The MA3-910 antigen is purified canine cardiac sarcoplasmic reticulum.

Target Information

This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol into the sarcoplasmic reticulum lumen, and is involved in regulation of the contraction/relaxation cycle. Mutations in this gene cause Darier-White disease, also known as keratosis follicularis, an autosomal dominant skin disorder characterized by loss of adhesion between epidermal cells and abnormal keratinization. Alternative splicing results in multiple transcript variants encoding different isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.