Thermo Fisher Scientific Vimentin Monoclonal Antibody (J144)
Vimentin 단백질을 인식하는 마우스 단클론 항체로, Western blot, IHC, ICC/IF 등 다양한 응용에 적합합니다. 사람, 쥐, 랫드, 개 시료에서 반응하며 -20°C 보관이 필요합니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 10 publications |
| Immunohistochemistry (IHC) | - | View 7 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:20 | View publications |
| Immunocytochemistry (ICC/IF) | 1:20 | View 5 publications |
| In vitro Assay (IV) | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Human, Mouse, Rat |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgM |
| Class | Monoclonal |
| Type | Antibody |
| Clone | J144 |
| Immunogen | Human rhabdosarcoma cell line JR1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not Determined |
| Storage Buffer | Ascites |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_326285 |
Product Specific Information
MA3-745 detects human, rat, and mouse vimentin and has been successfully used in HELA-S3, HEK-293, rat brain, and rat kidney cells.
It has been validated in Western blot, immunohistochemistry, and immunofluorescence applications.
By Western blot, this antibody detects an approximately 57 kDa protein representing vimentin from HELA-S3 cells.
The immunogen used was the human rhabdosarcoma cell line JR1.
Target Information
Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin.
It is involved in intracellular protein transport between the nucleus and plasma membrane.
Unlike other IFPs, vimentin is expressed with desmin during early cellular development and later replaced by tissue-specific IFPs.
Vimentin plays a role in steroid synthesis by acting as a storage network for cholesterol-containing lipid droplets.
Phosphorylation of vimentin leads to intermediate filament breakdown and cytoskeletal reorganization, facilitating cholesterol transport to mitochondria and increasing steroid synthesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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