Thermo Fisher Scientific Calsequestrin Monoclonal Antibody (VIIID12)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 64 publications 64 publications

Immunohistochemistry (IHC)

-

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20

-

Immunohistochemistry (PFA fixed) (IHC (PFA))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:50-1:500

View 3 publications 3 publications

Immunoprecipitation (IP)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Dog, Chicken, Human, Mouse, Pig, Rabbit, Rat

Published species

Chicken, Dog, Human, Mouse, Non-human primate, Pig, Rabbit, Rat, Rodent

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

VIIID12

Immunogen

Purified rabbit skeletal muscle sarcoplasmic reticulum. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Calsequestrin,uniProtId:P07221-1,ncbiNodeId:9986,antigenRange:1-395,antigenLength:395,antigenImageFileName:MA3-913_Calsequestrin_P07221-1_House_mouse.svg,antigenImageFileNamePDP:MA3-913_Calsequestrin_P07221-1_House_mouse_PDP.jpeg,sortOrder:3}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

PBS, ascites

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_325496

Product Specific Information

MA3-913 detects calsequestrin from human, mouse, rat, canine, porcine, chicken, and rabbit skeletal muscle tissues. This antibody recognizes calsequestrin in both type I (slow) and type II (fast) skeletal muscle tissues.

MA3-913 has been successfully used in Western blot procedures. By Western blot, this antibody detects a 63 kDa protein representing calsequestrin from canine skeletal muscle extracts. Higher molecular weight proteins are seen on the Western blot and are believed to be calsequestrin-like proteins found in the sarcoplasmic reticulum. The staining pattern yields double rows of fluorescent dots corresponding to triad pairs on either side of the Z-line.

The MA3-913 antigen is purified rabbit skeletal muscle sarcoplasmic reticulum.

Target Information

The sarcoplasmic reticulum (SR) is, in part, responsible for maintaining the level of intracellular calcium in cardiac and skeletal muscle by storing and releasing calcium. Several intralumenal SR calcium binding proteins have been identified, the most prominent of these is calsequestrin. Calsequstrin is a calcium binding protein known to sequester calcium accumulated in the sarcoplasmic reticulum of muscle cells during relaxation and is found discretely localized to the junctional and corbular (terminal cisternae) SR. Calsequestrin functions to localize calcium near the junctional face of the terminal cisternae from which calcium can be released into the cytosol via the ryanodine receptor. This protein is highly acidic and has a large capacity and moderate to low affinity for calcium.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.