Thermo Fisher Scientific GFAP Monoclonal Antibody (6F2)

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:50

-

Immunohistochemistry (Frozen) (IHC (F))

1:10-1:50

-

Immunocytochemistry (ICC/IF)

1:25-1:100

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Fish, Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

6F2

Immunogen

GFAP from human brain. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:GFAP,uniProtId:P14136-1,ncbiNodeId:9606,antigenRange:1-432,antigenLength:432,antigenImageFileName:MA1-35377_GFAP_P14136-1_House_mouse.svg,antigenImageFileNamePDP:MA1-35377_GFAP_P14136-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

25 µg/mL

Storage buffer

tissue culture supernatant

Contains

0.01M sodium azide

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_1074614

Product Specific Information

The antibody stains cells containing GFAP. The antibody only stains the 56kD GFAP band in immunoblots performed on crude brain extract. It reacts also with mouse GFAP

Target Information

GFAP (Glial fibrillary acidic protein) is a member of the class III intermediate filament protein family. GFAP is heavily and specifically expressed in astrocytes and certain astroglia of the central nervous system, in satellite cells of peripheral ganglia, and in non-myelinating Schwann cells of peripheral nerves. In addition, neural stem cells strongly express GFAP. Antibodies to GFAP are very useful as markers of astrocytic cells. In addition, many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes. GFAP is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing of the GFAP gene results in multiple transcript variants encoding distinct isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.