Thermo Fisher Scientific Collagen IV Monoclonal Antibody (MC4-HA)

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

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View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20

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Immunohistochemistry (Frozen) (IHC (F))

1:20

-

ELISA (ELISA)

Assay-dependent

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Product Specifications

Published species

Dog

Host/Isotype

Mouse / IgM

Class

Monoclonal

Type

Antibody

Clone

MC4-HA

Immunogen

Collagen type IV.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.2 mg/mL

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1073772

Product Specific Information

This antibody does not cross-react with Collagens I, II, III, or V.

For immunohistochemistry on frozen sections, fixation with acetone for 5 minutes at -20ºC is recommended. Immunohistochemistry on paraffin sections has worked, but is less effective than staining on frozen tissues.

Reconstitute with 1 mL of distilled water. Store reconstituted product at 4ºC.

Target Information

Collagen IV is a type of collagen protein that is a major component of the basement membrane in various tissues, including the kidneys, lungs, and skin. It forms a network-like structure that provides support and stability to the surrounding tissue. Collagen IV is composed of three alpha chains, which are encoded by the COL4A1, COL4A2, and COL4A3 genes. Mutations in these genes can lead to various genetic disorders, such as Alport syndrome and Goodpasture syndrome, which affect the kidneys and lungs, respectively.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.