Thermo Fisher Scientific NEUROG1 Monoclonal Antibody (OTI15A7), TrueMAB
인간 NEUROG1 단백질을 인식하는 Mouse IgG1 단클론 항체로, WB, IHC, ICC/IF, IP 등 다양한 응용에 적합. 무보존제, 동결건조 형태로 제공되며, 신경발생 연구 및 세포분화 조절 기전 분석에 유용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Immunoprecipitation (IP) | 2–4 µg/mg |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI15A7 |
| Immunogen | Full-length human NEUROG1 protein expressed in 293T cells transfected with human NEUROG1 expression vector |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Neurogenin 1 (NeuroG1) is a basic helix-loop-helix (bHLH) transcription factor involved in cell-type determination during development.
It plays dual roles in cell fate specification: promoting neurogenesis and inhibiting astrocyte differentiation.
NeuroG1 acts as a transcriptional activator to drive neurogenesis while preventing astrocyte differentiation by sequestering CREB-binding protein complexes and inhibiting STAT transcription factors essential for gliogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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