Thermo Fisher Scientific NEUROG1 Monoclonal Antibody (OTI5H10), TrueMAB
NEUROG1 단백질을 인식하는 mouse monoclonal 항체로, Western blot, IHC, ICC, IP 등 다양한 응용에 적합합니다. Human 시료에 반응하며, Lyophilized 형태로 제공됩니다. 신경분화 연구 및 세포 운명 결정 관련 연구에 유용합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Immunoprecipitation (IP) | 2–4 µg/mg |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI5H10 |
| Immunogen | Full-length protein expressed in 293T cells transfected with human NEUROG1 expression vector |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Neurogenin 1 (NEUROG1) is a basic helix-loop-helix (bHLH) transcription factor involved in cell fate determination during development.
It plays dual roles in neurogenesis and inhibition of astrocyte differentiation.
NeuroG1 promotes neuronal differentiation by acting as a transcriptional activator and inhibits astrocyte formation by sequestering the CREB-binding protein transcription complex and inhibiting STAT transcription factors essential for gliogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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