Thermo Fisher Scientific hnRNP M3-M4 Monoclonal Antibody (2A6)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:500

-

Immunohistochemistry (Frozen) (IHC (F))

1:10-1:500

-

Immunocytochemistry (ICC/IF)

1:10-1:500

-

Immunoprecipitation (IP)

1:10-1:500

-

Product Specifications

Species Reactivity

Bovine, Human, Mouse, Pig, Rabbit, Rat

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

2A6

Immunogen

M19 fusion protein containing full-length human protein. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:hnRNP M3-M4,uniProtId:P52272-1,ncbiNodeId:9606,antigenRange:1-730,antigenLength:730,antigenImageFileName:MA1-16674_hnRNP_M3-M4_P52272-1_House_mouse.svg,antigenImageFileNamePDP:MA1-16674_hnRNP_M3-M4_P52272-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.1% sodium azide

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Wet ice

RRID

AB_2120510

Product Specific Information

Suggested positive control: antigen standard for HNRNPM (transient overexpression lysate).

Target Information

Proteins that directly bind to nascent RNA polymerase II transcripts, the heterogenous nuclear ribonucleoproteins (hnRNPs), play an important role in both transcript-specific packaging and alternative splicing of pre-mRNAs. A group of abundant hnRNPs, the M1-M4 proteins, appear as a cluster of four proteins. The M proteins are pre-mRNA binding proteins in vivo, and they bind avidly to poly (G) and poly (U) RNA homopolymers in vitro. M proteins are members of the ribonucleoprotein consensus sequence family of RNA-binding proteins with greatest similarity to a hypothetical RNA-binding protein from Saccharomyces cerevisiae. The M proteins also possess an unusual hexapeptide-repeat region rich in methionine and arginine residues (MR repeat motif) that resembles a repeat in the 64kDa subunit of cleavage stimulation factor, which is involved in 3`-end maturation of pre-mRNAs. Proteins immunologically related to M exist in divergent eukaryotes ranging from human to yeast.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.