Thermo Fisher Scientific IL-5 Monoclonal Antibody (TRFK5), eBioscience
Mouse 및 Human IL-5에 반응하는 중화용 단클론 항체로, ELISA, ELISPOT, 중화 시험, IHC 및 Flow Cytometry 등에 사용 가능. 고순도(>90%) 및 낮은 응집도(<10%)를 가진 액상 형태로 제공되며, 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Flow Cytometry (Flow) | Assay-Dependent | 5 |
| ELISA | - | 9 |
| Neutralization (Neu) | Assay-Dependent | 2 |
| Functional Assay | Assay-Dependent | 1 |
| Radioimmune Assay (RIA) | - | 1 |
| Miscellaneous PubMed | - | 1 |
| Immunostaining (IS) | 1–4 µg/mL | - |
Product Specifications
| Category | Specification |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | TRFK5 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_468420 |
Additional Formats:
- eFluor 450 (Cat. 48-7052-82)
- Functional Grade (Cat. 16-7052-81)
- PE (Cat. 12-7052-82)
- Custom conjugation available upon request
Product Specific Information
Description:
The TRFK5 antibody reacts with mouse and human interleukin-5 (IL-5) and functions as a neutralizing antibody. Mouse IL-5 is a disulfide-linked homodimer composed of two 113 amino acid peptides, appearing as 32–34 kDa bands in SDS-PAGE. IL-5 is produced by T cells and is known as eosinophil-differentiating factor (EDF), B cell growth factor II (BCGFII), and T cell-replacing factor (TRF). IL-5 induces eosinophil differentiation and promotes survival and activation. In mice, IL-5 stimulates B cell proliferation and antibody production.
Applications Reported:
- ELISA capture
- ELISPOT capture
- Neutralization of IL-5 bioactivity
- Immunohistochemistry (IHC)
- Intracellular staining for flow cytometry
Applications Tested:
- Sandwich ELISA (mouse IL-5): capture with TRFK5, detection with biotin TRFK4 (13-7051), standard recombinant mouse IL-5 (14-8051). Recommended concentration: 1–4 µg/mL.
- Sandwich ELISA (human IL-5): capture with TRFK5, detection with biotin JES1-5A10 (13-7059), standard recombinant human IL-5 (14-80591). Recommended concentration: 1–4 µg/mL.
- Include standard curve (1000 pg/mL–8 pg/mL) per ELISA plate.
- Functional Grade purified TRFK5 tested for neutralization of IL-5 bioactivity.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
IL-5 is a cytokine that promotes growth and differentiation of B cells and eosinophils. It regulates eosinopoiesis, eosinophil maturation, and activation. Elevated IL-5 levels are associated with asthma and hypereosinophilic syndromes. The IL-5 receptor is a heterodimer sharing its beta subunit with IL-3 and CSF2/GM-CSF receptors. IL-5 forms a cytokine gene cluster on chromosome 5 with IL-4, IL-13, and CSF2, coordinated by long-range regulatory elements on 5q31.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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