Thermo Fisher Scientific IL-4 Monoclonal Antibody (8D4-8), eBioscience
Human IL-4에 특이적인 Mouse IgG1 monoclonal antibody로, ELISA 및 Flow Cytometry에 최적화되어 있음. 높은 순도(>90%)와 낮은 응집도(<10%)를 보유하며, 다양한 형광체 결합 형식 제공. 연구용으로만 사용 가능.
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Thermo Fisher Scientific IL-4 Monoclonal Antibody (8D4-8), eBioscience
Applications & Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 1 publication |
| Flow Cytometry (Flow) | - | View 15 publications |
| ELISA | 1–4 µg/mL | View 2 publications |
| Radioimmune Assays (RIA) | - | View 1 publication |
| T-Cell Activation (TCA) | - | View 1 publication |
| Affinity Purification (AP) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Cynomolgus monkey, Human, Mouse, Non-human primate, Rhesus monkey |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 8D4-8 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_468416 |
Additional Formats Available
Product Specific Information
Description:
The 8D4-8 antibody reacts with human interleukin-4 (IL-4), a 15–19 kDa cytokine secreted by Th2 cells.
Applications Reported:
The 8D4-8 antibody has been reported for use in capture of human IL-4 by ELISA and in intracellular staining for flow cytometric analysis.
Applications Tested:
- Tested as a capture antibody in sandwich ELISA for analysis of human IL-4.
- Used with biotinylated MP4-25D2 antibody (Product #13-7048-81) for detection and recombinant human IL-4 (Product #14-8049-80) as standard.
- Suitable range for ELISA capture: 1–4 µg/mL.
- Include a standard curve with recombinant IL-4 ranging 500 pg/mL – 4 pg/mL.
- Fluorochrome-conjugated 8D4-8 antibody tested for intracellular staining in flow cytometry at ≤0.5 µg/test.
- A test = antibody amount staining a sample in 100 µL volume (10⁵–10⁸ cells/test).
- Antibody titration recommended for optimal performance.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, serving as a ligand for the IL-4 receptor.
IL-4 and IL-13 share overlapping functions via receptor binding. STAT6 plays a key role in IL-4-mediated immune regulation.
The IL-4 gene is part of a cytokine cluster on chromosome 5q (with IL-3, IL-5, IL-13, CSF2).
IL-4 is closely located near IL-13 and IL-5, coordinated by long-range regulatory elements (~120 kb).
IL-4 delta 2 is a known splice variant.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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