Thermo Fisher Scientific CD123 Monoclonal Antibody (6H6), Super Bright 436, eBioscience
인간 CD123(IL-3 수용체 α사슬)을 인식하는 Mouse IgG1 단일클론 항체. Super Bright 436 형광체 결합으로 유세포분석(Flow Cytometry)에 최적화. Violet laser(405 nm)로 여기, 436 nm에서 방출. 미엘로이드 전구세포 등 다양한 세포 표면 발현 검출에 적합.
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Thermo Fisher Scientific CD123 Monoclonal Antibody (6H6), Super Bright 436, eBioscience™
Applications
- Flow Cytometry (Flow)
Tested Dilution: 5 µL (0.25 µg)/Test
Publications: View 10 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Super Bright™ 436, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 6H6 |
| Conjugate | Super Bright™ 436 |
| Excitation / Emission Max | 413 / 431 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2662727 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 532
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395
- Brilliant UV 615
- Brilliant UV 661
- Brilliant UV 737
- Brilliant UV 805
- Brilliant Violet 480
- Brilliant Violet 786
- eFluor 450
- FITC
- NovaFluor Blue 510
- PE
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 600
- Super Bright 645
- Super Bright 702
- Request custom conjugation
Product Specific Information
The 6H6 monoclonal antibody reacts with human CD123, the alpha chain of the IL-3 receptor. This 60–70 kDa transmembrane protein binds to IL-3 with low affinity by itself and, when associated with CD131 (common beta chain), binds IL-3 with high affinity.
CD123 is expressed by myeloid precursors, macrophages, dendritic cells, mast cells, basophils, and megakaryocytes.
This antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. Use at 5 µL (0.25 µg) per test (final volume 100 µL). Cell number can range from 10⁵ to 10⁸ cells/test.
Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. Recommended filter: 450/50 bandpass or equivalent. Ensure instrument compatibility.
When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401-42) to minimize non-specific polymer interactions.
Light Sensitivity: Protect from light; tandem dye is sensitive to photo-induced oxidation.
Fixation: Samples may be stored in IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days at 2–8°C in the dark with minimal impact on brightness and FRET efficiency.
Excitation: 405 nm
Emission: 436 nm
Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
IL-3Ra (Interleukin-3 receptor subunit alpha) is a member of the type I cytokine receptor family. It forms a heterodimer with the common beta subunit (CDw131) for signal transduction of Interleukin-3.
IL-3Ra is overexpressed on acute myeloid leukemia (AML) blasts compared with normal hematopoietic cells, making it a potential target for novel therapeutic agents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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