Thermo Fisher Scientific Cdc25C Monoclonal Antibody (TC-19)

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

-

Immunocytochemistry (ICC/IF)

Assay-dependent

-

Immunoprecipitation (IP)

1 µg

-

Product Specifications

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

TC-19

Immunogen

RBF/DnJ mice were immunized with recombinant human cdc25C fragment corresponding to amino acids 1-258 and fusing the splenocytes with NS-1 mouse myeloma cells. Epitope is known to be between amino acid residues 211-246.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_1955508

Product Specific Information

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

M-phase inducer phosphatase 3is an enzyme that in humans is encoded by theCDC25Cgene. This gene is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 (CDK1) and triggers entry into mitosis. Also, it is thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.