Thermo Fisher Scientific Phospho-AMPK alpha-1,2 (Thr172) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:300

-

Immunocytochemistry (ICC/IF)

5 µg/mL

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide sequence around phosphorylation site of threonine 183(L-R-T(p)-S-C)/threonine172(L- R-T(p)-S-C) derived from Human AMPKA1/AMPKA2. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:AMPK alpha-1,2,uniProtId:Q13131-1,ncbiNodeId:9606,antigenRange:170-174|181-185,antigenLength:559,antigenImageFileName:PA5-37821_AMPK_alpha-1,2_Q13131-1_Rabbit.svg,antigenImageFileNamePDP:PA5-37821_AMPK_alpha-1,2_Q13131-1_Rabbit_PDP.jpeg,sortOrder:1},{targetFamily:AMPK alpha-1,2,uniProtId:P54646-1,ncbiNodeId:9606,antigenRange:170-174|181-185,antigenLength:552,antigenImageFileName:PA5-37821_AMPK_alpha-1,2_P54646-1_Rabbit.svg,antigenImageFileNamePDP:PA5-37821_AMPK_alpha-1,2_P54646-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol, 0.5% BSA

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2554429

Product Specific Information

A suggested positive control for Western blot is C2C12 cells; suggested positive control for IHC is human lung carcinoma.

Target Information

The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.