Thermo Fisher Scientific Phospho-AMPK alpha-1,2 (Thr172) Polyclonal Antibody
AMPK α1/α2 Thr172 인산화 부위를 인식하는 Rabbit Polyclonal 항체로, WB, IHC, ICC 등 다양한 응용에 적합합니다. Human, Mouse, Rat 반응성을 가지며, PBS/glycerol buffer에 보관됩니다. 에너지 대사 연구 및 AMPK 신호전달 분석에 유용합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | View 1 publication |
| Immunohistochemistry (IHC) | – | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:100–1:300 | – |
| Immunocytochemistry (ICC/IF) | 5 µg/mL | View 1 publication |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide sequence around phosphorylation site of threonine 183 (L-R-T(p)-S-C) / threonine 172 (L-R-T(p)-S-C) derived from Human AMPKA1/AMPKA2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol, 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2554429 |
Product Specific Information
- Suggested positive control for Western blot: C2C12 cells
- Suggested positive control for IHC: Human lung carcinoma
Target Information
The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit and non-catalytic beta and gamma subunits. It functions as a key energy-sensing enzyme that monitors cellular energy status. Upon metabolic stress, AMPK becomes activated and phosphorylates acetyl-CoA carboxylase (ACC) and HMG-CoA reductase (HMGCR), regulating fatty acid and cholesterol biosynthesis. Mouse studies suggest this subunit influences insulin sensitivity and maintains myocardial energy homeostasis during ischemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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배송/결제/교환/반품 안내
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