Thermo Fisher Scientific Phospho-ERK1/ERK2 (Tyr205, Tyr222) Polyclonal Antibody
ERK1/2 단백질의 Tyr205 및 Tyr222 인산화 부위를 인식하는 Rabbit Polyclonal Antibody로, Western blot에 적합. Human, Mouse, Rat 반응성. 고순도 Affinity chromatography 정제, 1 mg/mL 농도, 안정적 PBS/glycerol buffer 제공.
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Applications
- Western Blot (WB): 1:500–1:1,000
Suggested positive control: JK cells
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 205/222 (K-G-Y(p)-T-K) derived from Human MAPK1/3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2554431 |
Target Information
ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells.
Many different stimuli, including growth factors, cytokines, virus infection, ligands for G protein-coupled receptors, and transforming agents, activate the ERK1/2 pathways.
Upon activation, ERKs phosphorylate cytoplasmic targets or migrate to the nucleus to activate transcription factors such as c-Fos and Elk-1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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