Thermo Fisher Scientific Phospho-PAK1/2/3 (Thr423, Thr402, Thr421) Polyclonal Antibody

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

-

Immunocytochemistry (ICC/IF)

1:100-1:200

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide sequence around phosphorylation site of threonine 423 /402/421 (R-S-T(p)-M-V) derived from Human PAK1/PAK2/PAK3. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PAK1/2/3,uniProtId:Q13153-1,ncbiNodeId:9606,antigenRange:402-423|421-425,antigenLength:545,antigenImageFileName:PA5-37819_PAK123_Q13153-1_Rabbit.svg,antigenImageFileNamePDP:PA5-37819_PAK123_Q13153-1_Rabbit_PDP.jpeg,sortOrder:1},{targetFamily:PAK1/2/3,uniProtId:O75914-1,ncbiNodeId:9606,antigenRange:402-423,antigenLength:559,antigenImageFileName:PA5-37819_PAK123_O75914-1_Rabbit.svg,antigenImageFileNamePDP:PA5-37819_PAK123_O75914-1_Rabbit_PDP.jpeg,sortOrder:2},{targetFamily:PAK1/2/3,uniProtId:Q13177-1,ncbiNodeId:9606,antigenRange:400-404|402-423,antigenLength:524,antigenImageFileName:PA5-37819_PAK123_Q13177-1_Rabbit.svg,antigenImageFileNamePDP:PA5-37819_PAK123_Q13177-1_Rabbit_PDP.jpeg,sortOrder:3}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2554427

Product Specific Information

A suggested positive control for IHC is human breast carcinoma; suggested positive control for ICC/IF is Hela cells.

Target Information

In mammals, there are several identified isoforms of p21-Activated Protein Kinases or PAKs: alpha-PAK (also known as PAK-1) and beta-PAK (also known as PAK-3) are mostly brain-specific, while gamma-PAK (also known as PAK-2) is expressed ubiquitously (Jakobi et al., 2003). Mutations of the gene coding for PAK-3 are associated wit X-linked mental retardation and recent work indicates that PAK-3 is a key regulator of synapse formation and plasticity in the hippocampus (Boda et al., 2004). PAK-3 is thought to play a key role in regulation of cell shape and motility as well as cell death (Jakobi et al., 2003; Walter et al., 1998). Autophosphorylation of Thr402 in the protein has been found to be essential for activation of PAK (Jakobi et al., 2000).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.