Thermo Fisher Scientific Phospho-PI3K p85/p55 (Tyr467, Tyr199) Polyclonal Antibody
PI3K p85/p55 단백질의 Tyr467 및 Tyr199 인산화 부위를 인식하는 Rabbit Polyclonal 항체. WB 및 ICC/IF에 사용 가능하며, 인간·마우스·랫트 반응성. 액상 형태, 1 mg/mL 농도, 친화 크로마토그래피 정제, 연구용 전용.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:1,000
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1:100–1:500
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 467 (L-Y(p)-E-E-Y) derived from Human PI3 Kinase p85/p55 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | -20°C |
| Shipping conditions | Wet ice |
| RRID | AB_2554428 |
Product Specific Information
A suggested positive control for Western blot is 293 cells.
Target Information
Phosphoinositide 3-kinase (PI3K, phospho PI3K) binds to activated (phosphorylated) protein-Tyr kinases through its SH2 domain and acts as an adapter mediating the association of the p110 catalytic unit to the plasma membrane.
Phospho PI3K is necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.
During insulin stimulation, it also binds to IRS-1. Growth factors and hormones trigger this phosphorylation event, which coordinates cell growth, cell cycle entry, cell migration, and cell survival.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지 파일명: PA5-37820_PI3K_p85p55_O00459-1_Rabbit.svg, PA5-37820_PI3K_p85p55_O00459-1_Rabbit_PDP.jpeg, PA5-37820_PI3K_p85p55_P27986-1_Rabbit.svg, PA5-37820_PI3K_p85p55_P27986-1_Rabbit_PDP.jpeg)
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