Thermo Fisher Scientific IL-4 Monoclonal Antibody (11B11), Brilliant Violet 480, eBioscience
마우스 IL-4를 인식하는 중화 모노클론 항체로, Brilliant Violet 480 형광체로 결합되어 흐름세포분석(Flow Cytometry)에 적합합니다. Th2 세포에서 분비되는 IL-4 검출에 사용되며, 0.25 µg/test 농도로 권장됩니다. 4°C 어두운 곳에 보관하며, 메탄올 고정에는 비호환입니다.
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Thermo Fisher Scientific IL-4 Monoclonal Antibody (11B11), Brilliant Violet 480, eBioscience™
Applications and Tested Dilution
- Application: Flow Cytometry (Flow)
- Tested Dilution: 0.25 µg/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rat / IgG1, kappa |
| Recommended Isotype Control | Rat IgG1 kappa Isotype Control (eBRG1), Brilliant Violet™ 480, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 11B11 |
| Conjugate | Brilliant Violet™ 480 |
| Excitation/Emission Max | 437 / 478 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Wet ice |
| RRID | AB_3074106 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- APC
- Brilliant UV 615
- Brilliant Violet 421
- Brilliant Violet 786
- Functional Grade
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Request custom conjugation
Product Specific Information
The 11B11 antibody reacts with mouse interleukin-4 (IL-4), a 14 kD cytokine, and functions as a neutralizing antibody. IL-4, also known as BCDF, BCGF-1, and BSF-1, is secreted mainly by activated Th2 and natural killer T cells, and promotes B cell proliferation and differentiation. It also induces Ig isotype switching and upregulates MHC class II and CD23 expression.
Applications Reported:
Suitable for intracellular staining followed by flow cytometric analysis.
Applications Tested:
Validated for intracellular staining of mouse Th2-polarized splenocytes using the Intracellular Fixation & Permeabilization Buffer Set (Product #88-8824-00).
Recommended usage ≤ 0.25 µg per test (100 µL final volume). Cell numbers from 10⁵–10⁸ cells/test are typical. Optimize antibody concentration for best performance.
Fluorochrome Information:
Brilliant Violet™ 480 emits at 479 nm and is designed for cytometers equipped with a 405 nm violet laser. Ensure compatibility before use.
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product #SB-4401-42) or Brilliant Stain Buffer™ (Product #00-4409-75) to reduce non-specific interactions.
Not compatible with methanol-based fixation.
Excitation: 440 nm
Emission: 479 nm
Laser: Violet Laser
BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.
Target Information
Interleukin-4 (IL-4) is an immune-modulatory cytokine produced by activated T cells, acting as a ligand for the IL-4 receptor. It shares functional overlap with IL-13 due to receptor binding. STAT6 mediates IL-4 signaling. The IL-4 gene is located on chromosome 5q near IL-13 and IL-5, forming a cytokine cluster regulated over ~120 kb. IL-4 delta 2 is a known splice variant.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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