
Thermo Fisher Scientific Mouse IgG2b kappa Isotype Control (eBMG2b), eFluor 570, eBioscience
단클론 Mouse IgG2b kappa 동형 대조 항체로, eFluor 570 형광 표지된 제품입니다. IHC(F/P), ICC/IF 등 다양한 면역염색에 적합하며, 실험 항체의 비특이적 결합 평가용으로 사용됩니다. 555/570 nm 형광 특성, 4°C 암소 보관.
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Thermo Fisher Scientific Mouse IgG2b kappa Isotype Control (eBMG2b), eFluor 570
Applications 및 Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1 µg/mL |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent |
| Immunocytochemistry (ICC/IF) | Assay-Dependent |
| Control (Ctrl) | Assay-Dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host/Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Isotype Control |
| Clone | eBMG2b |
| Conjugate | eFluor™ 570 |
| Excitation/Emission Max | 556/569 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2637335 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 488, 532, 647, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV 395–805 시리즈
- Brilliant Violet 421–786 시리즈
- eFluor 450, 506, 615, 660
- FITC, PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright 436, 600, 645, 702, 780
- Functional Grade
- Custom conjugation 요청 가능
Product Specific Information
Description:
Monoclonal mouse IgG2b is useful as an isotype control immunoglobulin.
Applications Reported:
This eBMG2b antibody has been reported for use in:
- Immunohistochemical staining of frozen and paraffin-embedded tissue sections
- Immunocytochemistry
Applications Tested:
Tested by immunocytochemistry on fixed and permeabilized cells.
Use isotype control at same concentration as experimental antibody.
Not validated for flow cytometry.
Filter Recommendation:
For eFluor™ 570 conjugates, use filters capturing 570 nm emission (e.g., Excitation 545/25, 565LP; Emission 605/70).
Standard Alexa Fluor™ 555 or TRITC filters are acceptable.
Excitation/Emission:
555 nm / 570 nm
Target Information
Mouse IgG2b is a subtype of immunoglobulin G produced by B cells in response to antigens.
It is the third most abundant IgG subtype in serum and plays a role in:
- Opsonization
- Complement activation
- Antibody-dependent cell-mediated cytotoxicity
Also involved in:
- Regulation of immune responses (T-cell activation, B-cell inhibition)
- Widely used in ELISA, Western blotting, immunohistochemistry, and immunotherapy/vaccine research.
For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.
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