Thermo Fisher Scientific CD127 Monoclonal Antibody (eBioRDR5), NovaFluor Red 710, eBioscience

Thermo Fisher Scientific CD127 Monoclonal Antibody (eBioRDR5), NovaFluor Red 710

Applications

• Flow Cytometry (Flow)

Tested Dilution

• 5 µL (0.2 µg)/test

Species Reactivity

• Human

Product Specifications

Additional Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 561, 647, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV/Violet series
• eFluor 450, 506, 660
• FITC, PE, PE-Cyanine series
• PerCP series
• Super Bright series
• NovaFluor Blue, Red, Violet, Yellow series
• Functional Grade
• Custom conjugation available

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Product Specific Information

Description:
The eBioRDR5 monoclonal antibody reacts with human CD127 (Interleukin-7 Receptor alpha). CD127 complexes with CD132 (common gamma chain) to form the IL-7 receptor (IL-7R), a type I glycoprotein (75–80 kDa) expressed on immature B cells, thymocytes, and most mature T cells. IL-7 binding triggers signal transduction via tyrosine kinase pathways including Jak/STAT.

CD127 expression is down-regulated on CD4+CD25+ regulatory T cells (Tregs). CD4+CD25+CD127^low cells are highly enriched for regulatory T populations expressing Foxp3 and showing suppressive activity.

Binding of eBioRDR5 to PBMCs is blocked by recombinant human IL-7.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated for use at 5 µL (0.2 µg) per test (100 µL final volume; 10⁵–10⁸ cells/test).

NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI). Use Invitrogen LIVE/DEAD Fixable Dead Cell stains instead.

CellBlox Plus Blocking Buffer (Product # C001T06F01)

• Required for optimal staining with NovaFluor conjugates
• Use 5 µL per sample (per 100 µL cell suspension)
• Add to antibody cocktails before mixing with cells

NovaFluor conjugates utilize Phiton™ technology with engineered fluorescent signatures minimizing spillover and spread.

Excitation: 639 nm
Emission: 710 nm
Laser: 633–640 nm (Red)

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Target Information

CD127 (Interleukin-7 receptor α) is a glycoprotein regulating lymphopoiesis. It forms an α/γ heterodimer receptor complex (IL7R) that mediates IL-7-dependent signaling. The γ chain activates transduction, while the α chain provides specificity. CD127 promotes proliferation of precursor B cells, thymocytes, T cell progenitors, and mature CD4+/CD8+ T cells.
Defects in CD127 are associated with severe combined immunodeficiency (SCID).

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For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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