Thermo Fisher Scientific CD127 Monoclonal Antibody (eBioRDR5), NovaFluor Red 700, eBioscience

Thermo Fisher Scientific CD127 Monoclonal Antibody (eBioRDR5), NovaFluor Red 700

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.2 µg)/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 561, 647, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 496, 563, 737, 805
• Brilliant Violet 421, 711, 786
• eFluor 450, 506, 660
• FITC
• Functional Grade
• NovaFluor Blue/Red/UV/Violet/Yellow variants
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Custom conjugation available

Product Specific Information

The eBioRDR5 monoclonal antibody reacts with human CD127 (Interleukin-7 Receptor alpha).
CD127 forms a complex with CD132 (common gamma chain) to create the IL-7 receptor (IL-7R), a type I glycoprotein (~75–80 kDa) expressed on immature B cells, thymocytes, and most mature T cells.

IL-7 binding triggers signal transduction through tyrosine kinase pathways such as Jak/STAT. IL-7 is essential for lymphocyte development and homeostatic T-cell proliferation. CD127 expression is down-regulated on CD4+CD25+ regulatory T cells, allowing improved identification of suppressive T reg populations.

Binding of eBioRDR5 to PBMCs is blocked by recombinant human IL-7 (Product #14-1079-80).

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: 5 µL (0.2 µg)/test; suitable for 10⁵–10⁸ cells/sample.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (PI, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assays.
• CellBlox Plus Blocking Buffer (Product #C001T06F01) is required for optimal staining.
  • Add 5 µL per sample or per 100 µL cell sample (10³–10⁸ cells).
• NovaFluor conjugates utilize Phiton™ technology for engineered fluorescent signatures with minimized spillover.

Excitation: 639 nm
Emission: 700 nm
Laser: 633–640 nm (Red)

Target Information

CD127 (Interleukin-7 receptor, IL7R) regulates lymphopoiesis via an alpha/gamma chain heterodimer.
The gamma(c) chain activates signal transduction, while the alpha chain mediates specific signaling.
CD127 promotes proliferation of precursor B cells, thymocytes, and mature CD4+/CD8+ T cells.
Dysfunction is associated with severe combined immunodeficiency (SCID).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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