Thermo Fisher Scientific GDF3 Polyclonal Antibody, Biotin, PeproTech

Thermo Fisher Scientific GDF3 Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
AAIPVPKLSC KNLCHRHQLF INFRDLGWHK WIIAPKGFMA NYCHGECPFS LTISLNSSNY AFMQALMHAV DPEIPQAVCI PTKLSPISML YQDNNDNVIL RHYEDMVVDE CGCG

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human GDF-3. Anti-Human GDF-3-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect hGDF-3 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human GDF-3 (500-P235) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant hGDF-3.

Western Blot:
To detect hGDF-3 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, the detection limit for Recombinant hGDF-3 is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Packaging:
500-P235BT-1MG will be provided as 2 × 500 µg.

Target Information

GDF3 is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. These proteins have a polybasic proteolytic processing site that produces a mature protein with seven conserved cysteine residues. Members of this family regulate cell growth and differentiation in embryonic and adult tissues. Expression studies suggest GDF3 may be involved in regulation of the adult lymphatic and erythroid systems and embryonic development.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지

(이미지 없음)