Thermo Fisher Scientific IGFBP7 Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein:
SSSDTCGPCE PASCPPLPPL GCLLGETRDA CGCCPMCARG EGEPCGGGGA GRGYCAPGME CVKSRKRRKG KAGAAAGGPG VSGVCVCKSR YPVCGSDGTT YPSGCQLRAA SQRAESRGEK AITQVSKGTC EQGPSIVTPP KDIWNVTGAQ VYLSCEVIGI PTPVLIWNKV KRGHYGVQRT ELLPGDRDNL AIQTRGGPEK HEVTGWVLVS PLSKEDAGEY ECHASNSQGQ ASASAKITVV DALHEIPVKK GEGAEL

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human IGF-BP7. Anti-Human IGF-BP7-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect hIGF-BP7 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human IGF-BP7 (500-P234) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant hIGF-BP7.

Western Blot:
To detect hIGF-BP7 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, the detection limit for Recombinant hIGF-BP7 is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Packaging:
500-P234BT-1MG will be provided as 2 × 500 µg.

Target Information

This gene encodes a member of the insulin-like growth factor (IGF)-binding protein (IGFBP) family. IGFBPs bind IGFs with high affinity, regulate IGF availability in body fluids and tissues, and modulate IGF binding to its receptors. This protein binds IGF-I and IGF-II with relatively low affinity and belongs to a subfamily of low-affinity IGFBPs. It also stimulates prostacyclin production and cell adhesion. Alternatively spliced transcript variants encoding different isoforms have been described for this gene, and one variant has been associated with retinal arterial macroaneurysm.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.