Thermo Fisher Scientific IGFBP5 Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein:
MLGSFVHCEP CDEKALSMCP PSPLGCELVK EPGCGCCMTC ALAEGQSCGV YTERCAQGLR CLPRQDEEKP LHALLHGRGV CLNEKSYREQ VKIERDSREH EEPTTSEMAE ETYSPKIFRP KHTRISELKA EAVKKDRRKK LTQSKFVGGA ENTAHPRIIS APEMRQESEQ GPCRRHMEAS LQELKASPRM VPRAVYLPNC DRKGFYKRKQ CKPSRGRKRG ICWCVDKYGM KLPGMEYVDG DFQCHTFDSS NVS.

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human IGF-BP5. Anti-Human IGF-BP5-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect hIGF-BP5 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human IGF-BP5 (500-P232) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant hIGF-BP5.

Western Blot:
To detect hIGF-BP5 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, the detection limit for Recombinant hIGF-BP5 is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Package Information:
500-P232BT-1MG will be provided as 2 × 500 µg.

Target Information

The insulin-like growth factor-binding proteins (IGFBPs) are a family of homologous proteins that have co-evolved with the IGFs. They serve as shuttle molecules for soluble IGFs and regulate IGF signaling. IGFBPs influence IGF bioavailability, concentration, and distribution in the extracellular environment, and also exhibit IGF-independent biological activity.
Seven IGFBPs have been described, differing in tissue distribution, half-lives, and modulation of IGF interactions. IGFBP5, secreted by myoblasts, plays a key role in muscle differentiation.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.