Thermo Fisher Scientific Bcl-2 Recombinant Mouse Monoclonal Antibody (rBCL2, 796)

Applications and Tested Dilution

Product Specifications

Product Specific Information

• For Immunohistochemistry (PFA-fixed): Incubate antibody for 30 minutes at room temperature.
• Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.

Target Information

BCL-2 is a key regulator of apoptosis, functioning to either inhibit or promote cell death. Members of the BCL-2 family dimerize to modulate apoptosis.

• Bag1 forms a heterodimer with BCL-2, enhancing its anti-apoptotic effect.
• Bax and Bak promote cytochrome c release from mitochondria, initiating apoptosis.
• Constitutive expression of BCL-2, such as translocation to the Ig heavy chain locus, is associated with follicular lymphoma.
• Neoplastic germinal centers in follicular lymphoma express high levels of BCL-2 alpha protein, unlike normal or hyperplastic centers.
• Two transcript variants of BCL-2 differ at their C-terminal ends.
• Overexpression of BCL-2 is linked to cancers such as B-cell lymphoma and prostate cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.