Thermo Fisher Scientific MHC Class II (I-A/I-E) Recombinant Rat Monoclonal Antibody (P7/7)
쥐 및 생쥐의 MHC Class II 항원을 인식하는 재조합 단클론 항체. IHC, Flow Cytometry, IP에 사용 가능. 다양한 rat haplotype 및 mouse I-A/I-E 분자에 교차 반응. Protein A로 정제된 액상 형태로 제공되며, 1 mg/mL 농도.
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Applications
- Immunohistochemistry (IHC): Assay-dependent
- Flow Cytometry (Flow): Assay-dependent
- Immunoprecipitation (IP): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse, Rat |
| Host / Isotype | Rat / IgG2b, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | P7/7 |
| Immunogen | This antibody was raised by immunising DA rats with PVG.R8 rat spleen cells. Splenocytes from immunised rats were then isolated, and fused to the myeloma line NSO/u to generate stable hybridomas. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
Product Specific Information
This antibody recognises the rat RT1Bu/Du MHC class II antigen, and cross-reacts with haplotypes c, d, l and n. It stains MHC class II molecules in several rat strains, including PVG, F334, LEW, and BN. However, it is not reactive with RT1a haplotype rats, including Wistar rats.
Additionally, this antibody cross-reacts with murine I-A and I-E molecules of all haplotypes tested (H-2b, d, f, k), making it suitable for all mouse strains.
Target Information
MHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on professional antigen-presenting cells such as macrophages, dendritic cells, and B cells. Before reaching the cell surface, MHC class II molecules bind endocytosed exogenous antigens, which are then presented to T cells.
The antigen-binding groove between the alpha and beta chains is open at both ends and accommodates peptides 15–24 amino acids long.
HLA and MHC antibodies are important tools in Immunopeptidomics, aiding in the identification and characterization of neoantigens using high-performance liquid chromatography coupled to tandem mass spectrometry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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