Thermo Fisher Scientific ARNT Polyclonal Antibody
ARNT 단백질을 인식하는 Thermo Fisher Scientific의 다클론 항체로, WB, IHC, IP 등 다양한 응용 가능. 인간 시료에 반응하며, 항원 친화 크로마토그래피로 정제됨. 액상 형태로 4°C 보관, 1mg/mL 농도 유지.
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Thermo Fisher Scientific ARNT Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (IHC) | 1:1,000–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000–1:5,000 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 1 and 50 of human Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Tris citrate/phosphate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections
- Based on 100% sequence identity, this antibody is predicted to react with Bovine
Target Information
HIF-1 beta is a series of aryl hydrocarbon receptor nuclear translocator (ARNT) gene products.
Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease, and chronic obstructive pulmonary disease.
HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis, regulated by posttranslational modification through prolyl hydroxylation.
It forms a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits. Under normoxic conditions, HIF-1 alpha undergoes hydroxylation and degradation, while under hypoxia, its stability and transcriptional activity increase.
In contrast, HIF-1 beta remains stable under both conditions.
Diseases associated with HIF-1 beta dysfunction include hypoxia and renal cell carcinoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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