Thermo Fisher Scientific CD215 (IL-15Ra) Monoclonal Antibody (eBioJM7A4), eBioscience
인간 IL-15 수용체 알파(IL-15Ra)를 인식하는 마우스 단클론 항체로, Flow Cytometry, Western Blot, Immunoprecipitation에 사용 가능. 비결합형(UNCONJUGATED)으로 제공되며, 고순도(>90%) 및 낮은 응집도(<10%)를 보유. 연구용으로만 사용.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-Dependent | - |
| Flow Cytometry (Flow) | 0.5 µg/test | 1 publication |
| Immunoprecipitation (IP) | Assay-Dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioJM7A4 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2865269 |
Additional Formats
Product Specific Information
Description:
The eBioJM7A4 monoclonal antibody reacts with the IL-15 receptor alpha chain (IL-15Rα). IL-15 and IL-2 are cytokines with overlapping but distinct biological effects. Their receptors share two subunits, IL-2Rβ and γ chains, essential for signal transduction. IL-15Rα is structurally related to IL-2Rα but binds IL-15 with approximately 1,000-fold higher affinity. Three differentially spliced IL-15Rα variants capable of high-affinity IL-15 binding have been identified in humans. The cytoplasmic domain of IL-15Rα, like IL-2Rα, is dispensable for mitogenic signaling, suggesting the α chain primarily confers high-affinity binding. At high concentrations, IL-15 can signal through IL-2Rβ and IL-2Rγ even without the α subunit.
Applications Reported:
Reported for use in flow cytometric analysis, immunoprecipitation, and Western blotting (WB).
Applications Tested:
Tested by flow cytometric analysis of LPS and IFNγ-stimulated human peripheral blood cells. Recommended ≤0.5 µg per test (100 µL final volume). Cell number may range from 10⁵–10⁸ cells/test. Antibody titration is advised for optimal performance.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Interleukin 15 receptor alpha (IL-15RA) is a cytokine receptor that binds IL-15 with high affinity. IL-15 regulates T and NK cell activation and proliferation. IL-15 and IL-2 receptors share IL-2Rβ and IL-2Rγ subunits, with IL-15RA structurally related to IL-2RA. Unlike IL-2RA, IL-15RA binds IL-15 independently of other subunits. IL-15RA enhances cell proliferation and expression of apoptosis inhibitors Bcl-xL and Bcl-2.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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