Thermo Fisher Scientific IL-13 Monoclonal Antibody (eBio13A), eBioscience

Thermo Fisher Scientific IL-13 Monoclonal Antibody (eBio13A), eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats Available

• Alexa Fluor 488
• APC-eFluor 780
• Brilliant UV 737
• Brilliant UV 805
• eFluor 450
• eFluor 660
• Functional Grade
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation available

Product Specific Information

Description:
The eBio13A antibody reacts with mouse IL-13, a cytokine mainly produced by Th2 cells and antigen-primed CD8 T cells. IL-13 plays a critical role in allergic inflammation, parasite clearance, and tumor regulation. In humans, IL-13 contributes to B cell isotype switching and down-modulates macrophage activity by reducing pro-inflammatory cytokines (IL-1, IL-6, IL-8, IL-10, IL-12).

Applications Reported:
Useful for ELISA and ELISPOT capture assays, and intracellular staining in flow cytometry.

Applications Tested:
Validated as a capture antibody for sandwich ELISA and ELISPOT analysis of mouse IL-13, in combination with biotinylated eBio1316H antibody (13-7135) for detection and recombinant mouse IL-13 (14-8131) as standard. Recommended ELISA capture range: 1.0–4.0 µg/mL. Include a standard curve (1000 pg/mL–8.0 pg/mL) in each ELISA plate.

Functional Grade Purified eBio1316H antibody (16-7135) is recommended for in vitro blocking studies.

Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered

Target Information

IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells. It regulates inflammatory and immune responses by down-regulating macrophage activity and inhibiting pro-inflammatory cytokine production. IL-13 is implicated in allergen-induced asthma via mechanisms independent of IgE and eosinophils, and influences B cell maturation, differentiation, and IgE isotype switching. It shares structural similarity with IL-3, IL-4, IL-5, and GM-CSF, forming a cytokine gene cluster on chromosome 5q near IL4.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.