Thermo Fisher Scientific MHC Class I (H-2Db) Monoclonal Antibody (28-14-8), NovaFluor Red 755, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Available Formats

• Unconjugated
• APC
• Biotin
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 510, 585, 610-70S, 660-120S, 690
• NovaFluor Red 710
• NovaFluor Yellow 570, 660, 730, 755
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Custom conjugation available

Product Specific Information

Description:
The 28-14-8 monoclonal antibody reacts with mouse MHC class I, H-2D^b, and cross-reacts with H-2L^d, H-2D^q, and/or H-2L^q. Binding to H-2L^d alpha 3 domain is not dependent on beta2-microglobulin.

Each product includes one vial of NovaFluor conjugate and one vial of CellBlox Plus Blocking Buffer.

Applications Reported:
Reported for use in flow cytometric analysis.

Applications Tested:
Validated by flow cytometric analysis of mouse splenocytes. Recommended ≤ 0.6 µg/test in 100 µL final volume. Cell number may vary (10^5–10^8 cells/test). Titrate for optimal performance.

Important Notes:

• NovaFluor dyes are incompatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains instead.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining.
• Add 5 µL of CellBlox Plus per sample or per 100 µL of single-color control sample.

Technology:
NovaFluor conjugates utilize Phiton™ technology, based on nucleic acid dye structures enabling engineered fluorescent signatures with minimized spillover and spread.

Excitation: 636 nm
Emission: 756 nm
Laser: 633–640 nm (Red)

Target Information

MHC class I alloantigen involved in antigen presentation to CD3/TCR and CD8-positive T cells.
HLA and MHC antibodies are key tools in immunopeptidomics, supporting neoantigen identification and characterization via LC-MS/MS.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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