Thermo Fisher Scientific MHC Class I (H-2Db) Monoclonal Antibody (28-14-8), NovaFluor Red 755, eBioscience

Thermo Fisher Scientific MHC Class I (H-2Db) Monoclonal Antibody (28-14-8), NovaFluor Red 755

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.6 µg/test

Product Specifications

Additional Formats

• Unconjugated
• APC
• Biotin
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 510 / 585 / 610-70S / 660-120S / 690
• NovaFluor Red 710
• NovaFluor Yellow 570 / 660 / 730 / 755
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Custom conjugation available

Product Specific Information

The 28-14-8 monoclonal antibody reacts with mouse MHC class I, H-2D^b, and cross-reacts with H-2L^d, H-2D^q, and/or H-2L^q. Binding to the alpha 3 domain of H-2L^d is independent of beta2-microglobulin.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes at ≤0.6 µg per test (100 µL final volume).

Usage Notes:

• Optimize antibody titration for best assay performance.
• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Recommended viability dye: Invitrogen LIVE/DEAD Fixable Dead Cell stains.
• Use CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) for optimal staining.
• Add 5 µL per sample to antibody cocktails or single-color controls.

Technology:
NovaFluor conjugates utilize Phiton™ technology with engineered nucleic acid dye structures for optimized fluorescence performance and minimal spectral spillover.

Excitation: 636 nm
Emission: 756 nm
Laser: 633–640 nm (Red)

Target Information

MHC Class I alloantigen involved in antigen presentation to CD3/TCR and CD8-expressing T cells.
HLA and MHC antibodies are essential in Immunopeptidomics for neoantigen identification and characterization via LC-MS/MS.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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