Thermo Fisher Scientific MHC Class I (H-2Db) Monoclonal Antibody (28-14-8), NovaFluor Blue 690, eBioscience

Thermo Fisher Scientific MHC Class I (H-2Db) Monoclonal Antibody (28-14-8), NovaFluor Blue 690

Applications

• Flow Cytometry

Tested Dilution: 1.0 µg/test

Product Specifications

Additional Formats Available

• Unconjugated
• APC
• Biotin
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 510
• NovaFluor Blue 585
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Red 710
• NovaFluor Red 755
• NovaFluor Yellow 570
• NovaFluor Yellow 660
• NovaFluor Yellow 730
• NovaFluor Yellow 755
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Custom conjugation available

Product Specific Information

The 28-14-8 monoclonal antibody reacts with mouse MHC Class I, H-2D^b, and cross-reacts with H-2L^d, H-2D^q, and/or H-2L^q. Binding to the alpha 3 domain of H-2L^d is independent of beta2-microglobulin.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes at ≤1 µg per test (100 µL final volume). Cell number per test may range from 10^5 to 10^8. Optimize antibody titration for best performance.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes such as propidium iodide, 7-AAD, or DAPI.
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability analysis.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining with NovaFluor conjugates.
• Add 5 µL of CellBlox Plus per sample (10^3–10^8 cells) before combining with cells or antibody cocktails.

NovaFluor Technology:
Based on Phiton™ nucleic acid dye structures, engineered for minimal spillover and improved spectral separation.
Excitation: 494 nm; Emission: 585 nm; Laser: 488 nm (Blue).

Target Information

MHC Class I alloantigen involved in antigen presentation to CD3/TCR and CD8 T cells.
HLA and MHC antibodies are essential tools in Immunopeptidomics for identifying neoantigens via LC-MS/MS.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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