Thermo Fisher Scientific MHC Class I (H-2Db) Monoclonal Antibody (28-14-8), NovaFluor Red 710, eBioscience

Thermo Fisher Scientific MHC Class I (H-2Db) Monoclonal Antibody (28-14-8), NovaFluor Red 710

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.6 µg/test

Product Specifications

Additional Formats

• Unconjugated
• APC
• Biotin
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 510 / 585 / 610-70S / 660-120S / 690
• NovaFluor Red 755
• NovaFluor Yellow 570 / 660 / 730 / 755
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Request custom conjugation available

Product Specific Information

The 28-14-8 monoclonal antibody reacts with mouse MHC class I, H-2D^b, and cross-reacts with H-2L^d, H-2D^q and/or H-2L^q. Binding to the alpha 3 domain of H-2L^d is not dependent on beta2-microglobulin.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes, ≤1 µg per test (100 µL final volume, 10^5–10^8 cells/test). Titrate for optimal performance.

Compatibility Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assays.

CellBlox Plus Blocking Buffer (Cat. No. C001T06F01):

• Enhanced formulation for optimal staining.
• Add 5 µL per sample to antibody cocktails before mixing with cells.
• For single-color controls: 5 µL per 100 µL cell sample (10^3–10^8 cells).

NovaFluor Technology:
NovaFluor conjugates utilize Phiton™ technology with engineered fluorescent signatures designed to minimize spectral spillover and spread.
Excitation: 639 nm; Emission: 710 nm; Laser: 633–640 nm (Red).

Target Information

MHC Class I alloantigen involved in antigen presentation to CD3/TCR and CD8-expressing T cells.
HLA and MHC antibodies are essential in immunopeptidomics for neoantigen identification and characterization via LC-MS/MS.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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