Thermo Fisher Scientific PDE6 Beta Polyclonal Antibody, FITC
PDE6 Beta 단백질을 인식하는 FITC 표지 폴리클로날 항체로, WB, IHC, ICC/IF, ELISA 등 다양한 분석에 적용 가능. 토끼 유래 IgG 항체이며, Bovine, Human, Mouse, Pig, Rat에 반응. 액상 형태로 -20°C 어둠 속 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (IHC) | 1:50–1:250 |
| Immunocytochemistry (ICC/IF) | 1:50–1:250 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
| Immunomicroscopy (IM) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Human, Mouse, Pig, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic cyclic peptide common to all PDE6 Beta variants |
| Conjugate | FITC (Fluorescein) |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer, pH 7.4–7.8, with 0.5% BSA, 30% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The second messengers cAMP and cGMP are key regulatory molecules involved in a wide variety of signal transduction pathways. Their levels are regulated by synthesis via nucleotide cyclases and hydrolysis by cyclic nucleotide phosphodiesterases (PDEs). PDEs form a superfamily of enzymes that convert 3′,5′-cyclic nucleotides to the corresponding nucleoside 5′-monophosphates.
PDE6 functions as the effector enzyme in the G protein-mediated visual signal transduction cascade. It consists of rod and cone-specific catalytic subunits (α′ for cone, α and β for rod), an inhibitory subunit γ, and a δ subunit of unknown function. The catalytic core comprises α′/α′ homodimers in cones and α/β heterodimers in rods. The C-termini of both catalytic and inhibitory subunits undergo methylation, myristoylation, and prenylation, which are essential for proper complex assembly and membrane association.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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