Thermo Fisher Scientific Phospho-Progesterone Receptor (Ser190) Monoclonal Antibody (1154)

Applications

Tested Dilution

Publications

Western Blot (WB)

4 µg/mL

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

2-4 µg/mL

-

Immunocytochemistry (ICC/IF)

1:10-1:100

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

-

Immunoprecipitation (IP)

Assay-dependent

-

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1154

Immunogen

Synthetic phosphopeptide corresponding to residues V(184) L P R G L S(p) P A R Q L L(196) of human Progesterone Receptor. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Progesterone Receptor,uniProtId:P06401-1,ncbiNodeId:9606,antigenRange:185-196,antigenLength:933,antigenImageFileName:MA1-413_Progesterone_Receptor_P06401-1_House_mouse.svg,antigenImageFileNamePDP:MA1-413_Progesterone_Receptor_P06401-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

purified

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_325315

Product Specific Information

MA1-413 detects phospho-progesterone receptor (pSer190) from human samples.

MA1-413 has been successfully used in Western blot, immunohistochemistry, ELISA, immunofluorescence, and immunoprecipitation procedures. By Western blot, this antibody detects ~81 and ~116 kDa proteins representing phospho-progesterone receptor (pSer190) A and B from T47D cell and human breast carcinoma extracts. Immunohistochemical staining of phospho-progesterone receptor (pSer190) (paraffin samples) can be performed using MA1-413.

The MA1-413 immunogen is a synthetic peptide corresponding to residues V(184) L P R G L S(p) P A R Q L L(196) of human Progesterone Receptor.

Target Information

The progesterone receptor (PR) is a member of the steroid family of nuclear receptors. The PR mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. PR is found as a 94 kDa protein (Form A) or a 120 kDa protein (Form B) due to the use of alternative translation initiation sites. PR-B is the transcriptionally active form and is responsible for activating genes for the maintenance of the endometrium, maintenance of pregnancy, and inhibition of ovulation. PR-A is identical to PR-B except for a 165 amino acid deletion at the N-terminus. This deletion exposes a 140 amino acid inhibitory domain (ID) that acts as a repressor of steroid hormone transcriptional activity. In its inactive state, PgR forms a multiprotein complex which includes heat shock proteins and immunophins. Upon binding of progesterone hormone to its receptor, there is a conformational change that allows dimerization and binding of the receptor to progesterone response elements (PRE) sequences, resulting in activated transcription. A Null mutation in the PGR gene leads to pleiotrophic reproductive abnormalities.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.