Thermo Fisher Scientific Caspase 8 Monoclonal Antibody (90A992)
Caspase 8 단백질을 검출하는 Mouse IgG1 단일클론 항체로, Western blot, IHC, Flow cytometry에 적합합니다. 인간 및 영장류 시료에서 반응하며, 단백질 G 정제된 액상 형태로 제공됩니다. 세포사멸 연구용으로 활용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.5–2 µg/mL | View 6 publications |
| Immunohistochemistry (IHC) | – | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 4 µg/mL | – |
| Flow Cytometry (Flow) | 0.1–0.5 µg/mL | – |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Non-human primate |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 90A992 |
| Immunogen | Amino acids 360–385 of the human Caspase 8 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1071016 |
Product Specific Information
MA1-41280 detects Caspase-8 in human, Rhesus monkey, and chimpanzee samples.
Suggested positive controls: Jurkat cells, antigen standard for CASP8 (transient overexpression lysate), Jurkat whole cell lysate.
Target Information
Caspase 8 (CASP8) is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution phase of cell apoptosis.
Caspases exist as inactive proenzymes composed of a pro-domain, a large protease subunit, and a small protease subunit. Activation requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of large and small subunits.
Caspase 8 is involved in programmed cell death induced by Fas and various apoptotic stimuli. Its N-terminal FADD-like death effector domain suggests interaction with Fas-interacting protein FADD. Caspase 8 has been detected in insoluble fractions of affected brain regions from Huntington disease patients, implicating a role in neurodegenerative disease.
Activated Caspase 8 triggers downstream caspases, including Caspase 9, leading to apoptosis. Overexpression induces apoptosis, which can be blocked by ICE family inhibitors. Multiple alternatively spliced transcript variants exist, though not all full-length sequences are determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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