Thermo Fisher Scientific NFkB p50 Monoclonal Antibody (2J10D7)
NFkB p50 단백질을 인식하는 Mouse 모노클로날 항체로, WB, IHC, Flow Cytometry에 사용 가능. Human, Rabbit, Rat 반응성. Protein G 정제, 1 mg/mL 농도, PBS buffer에 0.05% sodium azide 포함. 세포 성장 및 염증 관련 연구에 적합.
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Thermo Fisher Scientific NFkB p50 Monoclonal Antibody (2J10D7)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 2–5 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5 µg/mL |
| Flow Cytometry (Flow) | Assay-dependent |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Rabbit, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2J10D7 |
| Immunogen | A portion of amino acids 150–200 of human NF-kB (p50) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1077242 |
Product Specific Information
This antibody is predicted to react with mouse and rat samples based on sequence homology.
Suggested positive control: antigen standard for NFKB1 (transient overexpression lysate), HeLa whole cell extract, HeLa cell lysate.
Target Information
NFkB is a nuclear transcription factor activated by various stimuli such as cytokines, UV radiation, stress, injury, and microbial products. It regulates cellular events including growth, differentiation, proliferation, apoptosis, and inflammation.
NFKB1 (p50) is a 50 kDa subunit of NF-kB, part of the Rel protein family, synthesized as a p105 precursor containing nuclear localization, DNA-binding, and dimerization domains.
p50 forms homodimers or heterodimerizes with p65, forming the functional NFkB complex. The NFkB complex is mainly cytoplasmic in an inactive state bound to I-kappa-B. Upon phosphorylation of I-kappa-B, NFkB translocates to the nucleus to regulate gene expression.
Phosphorylation of the p50 subunit at Ser-337 enhances DNA-binding efficiency. NFkB plays roles in AIDS, cancer metastasis, and rheumatoid arthritis pathogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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