Thermo Fisher Scientific NFkB p50 Monoclonal Antibody (2J10D7)

Applications

Tested Dilution

Publications

Western Blot (WB)

2-5 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

5 µg/mL

-

Flow Cytometry (Flow)

Assay-Dependent

-

Product Specifications

Species Reactivity

Human, Rabbit, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

2J10D7

Immunogen

A portion of amino acids 150-200 of human NF-kB (p50) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:NFkB p50,uniProtId:P19838-1,ncbiNodeId:9606,antigenRange:150-200,antigenLength:968,antigenImageFileName:MA1-41314_NFkB_p50_P19838-1_House_mouse.svg,antigenImageFileNamePDP:MA1-41314_NFkB_p50_P19838-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1077242

Product Specific Information

This antibody is predicted to react with mouse and rat samples based on sequence homology.

Suggested positive control: antigen standard for NFKB1 (transient overexpression lysate), Hela whole cell extract, HeLa cell lysate.

Target Information

NFkB is a nuclear transcription factor activated by various extra and intracellular stimuli such as cytokines, UV radiation, stress, in injury, and by bacterial and viral products. NFkB is involved in regulation of various cellular events including cell growth, differentiation, proliferation, apoptosis and inflammation. NFKB1 (p50) is a 50KDa functional sub-unit of NF-kB and a member of Rel protein family which is synthesized as a p105 precursor protein and consists of an N-terminal conserved RHD-region containing nuclear localization signal, DNA-binding and dimerization domains. NFKB1 (p50) forms homodimers or heterodimerizes with p65, forming the functional NFkB factor. Further, NFKB1 (p50) directs the nuclear translocation of NF-kB and is instrumental in its DNA-binding. The NFkB complex is expressed in most cell types, and is primarily found in the cytoplasm in an inactive state in association with I-kappa-B. Phosphorylation of I-kappa-B releases the complex, allowing translocation to the nucleus and modification of gene expression. Phosphorylation of the p50 subunit at the Ser-337 residue has been shown to significantly increase DNA binding efficiency of the NFkB protein complex. The pathological role of NFkB has been suggested in AIDS, hematogenic cancer cell metastasis and rheumatoid arthritis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.